Lush Prize 1R Network


The Lush Prize 1R Network was created to help winners and friends of the Lush Prize collaborate on projects working towards the replacement of animal use in chemical safety assessment.

Like the Lush Prize, the Network is for people who take the view that the 3R paradigm (which has the goal of reducing, refining or replacing animal tests) should be superceded by a 1R paradigm which has the goal of the complete replacement of all animal-based tests with non-animal alternatives.

This is because, since the 3Rs was first proposed in the 1950s, animal tests have become viewed as increasingly unreliable predictors for human outcomes as well as harmful to sentient creatures.

The Network’s primary activities are:
1. Arranging an annual conference with the Lush Prize team;
2. providing an online forum for free contact and collaboration between members;
3. publishing a quarterly email newsletter for members;
4. curating a toolkit of documents and links to useful 1R resources.


Network Forum

Some of the most significant impacts of the Lush Prize have been made through independent collaborations between winners, judges or Lush team members.

The Forum provides an online space to enable more of this independent collaboration between members which further the network’s mission.  We hope to see more collaborations from members across different prize years and prize categories.

Access to the Forum is restricted to members. Membership is currently by invitation only, although we will have an enquiry form available soon. If you have an account you can sign in here.

The mission of the forum is to:

  • Collaborate with other members to achieve a future where animals are no longer used for chemical safety assessment;
  • raise public awareness of the superiority of animal-free approaches (New Approach Methodologies or NAMs) to chemical safety testing across a variety of endpoints;
  • engage with relevant stakeholders around the value of replacing a 3Rs paradigm with a 1R approach;
  • support members in their efforts to replace any animal-based components used in their research.


Network Toolkit

1. Evidence supporting the network’s mission

Current EU regulatory requirements for the assessment of chemicals and cosmetic products: challenges and opportunities for introducing new approach methodologies (Pistollato, F. et al. 2021)

‘Animal Experimentation: Working Towards a Paradigm Change’ – Open access book
(Editors: Kathrin Herrmann and Kimberley Jayne. 2019)

‘Global View of Animal Experiments’ – a 2014 report by Lush Prize

NRC Toxicity testing in the 21st century: A vision and a strategy’ (2007)

‘Accelerating the Growth of Human Relevant Life Sciences – A White Paper‘ (Alliance for Human Relevant Science 2020)

Harnessing the power of novel animal-free test methods for the development of COVID-19 drugs and vaccines’ (Hartung, Leist et al. 2020).

Animals Used for Scientific Purposes (European Commission). This is a key information portal from the European Commission which is updated regularly. It includes 3Rs initiatives, but also 1R resources directly relevant to the LPARN.

Modelling the Pathogenesis of COVID-19 using the Adverse Outcome Pathway Framework (CIAO Project)


2. Relevant documentation/lists of New Approach Methodologies (NAMs)

This section provides links to key reference documents (e.g. lists of currently accepted NAMs or guidance documents on NAM use) according to international regulations (e.g. REACH (EU); TSCA (USA).

List of Alternative Test Methods and Strategies (or New Approach Methodologies [NAMs]’relevant to the Toxic Substances Control Act (TSCA); US Environmental Protection Agency (updated Feb 2021)

Read Across Assessment Framework for REACH Regulation (Registration, Evaluation and Authorisation of CHemicals). European Chemicals Agency (ECHA, 2017)


3. Databases of replacement technologies

The NAT Database (Non-Animal Technologies)

FCS-Free Database

Collaborative Adverse Outcome Pathway Wiki

AOP Knowledge Base

QSAR Toolbox

EURL ECVAM  knowledge base with descriptions of over 900 non-animal models for neurodegenerative diseases (provides link to download spreadsheet/database from JRC Catalogue)

EURL ECVAM knowledge base with descriptions of over 900 non-animal models for breast cancer research (provides link to download spreadsheet/database from JRC Catalogue)

EURL ECVAM knowledge base with descriptions of nearly 300 non-animal models used for respiratory disease research (provides link to download spreadsheet/database from JRC Catalogue)


4. Video gallery